Haynes Lab:Notebook/CRISPR Editing/2016/06/16

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06/16/2016

Plate 10

16.06.16 qPCR Plate 10 screen shot.png



P313/311 master mix

' 1 well 40
2x SYBR mix 7.5 300
750nmol F/R primer mix 3 120
PCR water 2.5 100
total 13 520


Pset 3 master mix

' 1 well 58
2x SYBR mix 7.5 435
750nmol F/R primer mix 3 174
PCR water 2.5 145
total 13 754



Plate 11

16.06.16 qPCR plate 11 screen shot.png


P313/311 master mix

' 1 well 51
2x SYBR mix 7.5 382.5
750nmol F/R primer mix 3 153
PCR water 2.5 127.5
total 13 663

Primer set 3 master mix

' 1 well 27
2x SYBR mix 7.5 202.5
750nmol F/R primer mix 3 81
PCR water 2.5 67.5
total 13 351