06/13/2016
qPCR plate 9
all L and G 25 for H3K27me3 for the new g031/34 primer set P313/311
try new off target site for FLAG antibody binding, located at the end of the luciferase transgene, just in case something weird is happening at the GAPDH site or with those primers
master mix for P313/311
'
|
1 well
|
85
|
2x SYBR mix |
7.5 |
637.5
|
750nmol F/R primer mix |
3 |
255
|
PCR water |
2.5 |
212.5
|
total |
13 |
1105
|
master mix for P157/158
'
|
1 well
|
11
|
2x SYBR mix |
7.5 |
82.5
|
750nmol F/R primer mix |
3 |
33
|
PCR water |
2.5 |
27.5
|
total |
13 |
143
|
|