Haynes Lab:Notebook/CRISPR Editing/2016/06/13

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06/13/2016 qPCR plate 9 all L and G 25 for H3K27me3 for the new g031/34 primer set P313/311 try new off target site for FLAG antibody binding, located at the end of the luciferase transgene, just in case something weird is happening at the GAPDH site or with those primers master mix for P313/311 ' 1 well 85 2x SYBR mix 7.5 637.5 750nmol F/R primer mix 3 255 PCR water 2.5 212.5 total 13 1105 master mix for P157/158 ' 1 well 11 2x SYBR mix 7.5 82.5 750nmol F/R primer mix 3 33 PCR water 2.5 27.5 total 13 143