05/31/2016
qPCR for ChIP
Plate 2
Dropping Primer set 3 because the input calls were around 39 so there's no chance we'd see any pulldown DNA. Josh gave me new primers against GAPDH promoter he's been using for his ChIPs. Will test those for one input set on this plate.
Also adding wells with high variability between technical replicates. All the Luc14 samples on this plate are re-runs.
Primer set 1 MM, 39 wells
| '
|
1 well
|
42
|
| 2x SYBR mix |
7.5 |
315
|
| 750nmol F/R primer mix |
3 |
126
|
| PCR water |
2.5 |
105
|
| total |
13 |
546
|
Primer set 2 MM, 33 wells
| '
|
1 well
|
36
|
| 2x SYBR mix |
7.5 |
270
|
| 750nmol F/R primer mix |
3 |
108
|
| PCR water |
2.5 |
90
|
| total |
13 |
468
|
Primer set 3 MM, 12 wells
| '
|
1 well
|
14
|
| 2x SYBR mix |
7.5 |
105
|
| 750nmol F/R primer mix |
3 |
42
|
| PCR water |
2.5 |
35
|
| total |
13 |
182
|
Plate 3
Primer set 1 MM, 21 wells
| '
|
1 well
|
24
|
| 2x SYBR mix |
7.5 |
180
|
| 750nmol F/R primer mix |
3 |
72
|
| PCR water |
2.5 |
60
|
| total |
13 |
312
|
Primer set 2 MM, 57 wells
| '
|
1 well
|
60
|
| 2x SYBR mix |
7.5 |
450
|
| 750nmol F/R primer mix |
3 |
180
|
| PCR water |
2.5 |
150
|
| total |
13 |
780
|
Plate 4
Run by Josh Cutts, the best
Primer set 3 MM, 84 wells
| '
|
1 well
|
87
|
| 2x SYBR mix |
7.5 |
652.5
|
| 750nmol F/R primer mix |
3 |
261
|
| PCR water |
2.5 |
217.5
|
| total |
13 |
1131
|
13ul master mix into each well, 2ul of sample into each well, following guide below:
| 
Project name
Main project page
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