Haynes Lab:Notebook/CRISPR Editing/2016/05/30

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05/30/2016

qPCR for ChIP!
Primer sets

Primer set Target F primer R primer amplicon length
1 g025 P331 P329 205
2 g031, g034 P308 P160 202
3 GAPDH P203 P204



Plate 1
Luc14

Reps 1-3
H3K27me3, IgG, input
Primer set 1, Primer set 2, Primer set 3


16.05.30 qPCR Plate 1 screen shot.png
Primer master mix for primer sets 1, 2, and 3

' 1 wells 34
2x SYBR mix 7.5 255
750nmol F/R primer mix 3 102
PCR water 2.5 85
total 13 442






Redo nested PCR of the KAH228/g034 experiments
Did it with the wrong primers the first time, need to redo with P149/176.



Redo nested PCR of the Luc14 g034 samples
Did it with the wrong primers the first time, need to redo with P149/176.