Haynes Lab:Notebook/CRISPR Editing/2016/05/21

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05/21/2015

Purified nested PCR for Luc14 g025 and g048. Stacked the previously purified g048 DNA

Cell type gRNA rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul H20
Luc14 g025 1 0.035 0.02 1.792 35.279 11.33818986 1.5 2.161810142
Luc14 g025 2 0.02 0.011 1.864 19.713 20.29117841 1.5 -6.79117841
Luc14 g025 3 0.019 0.01 1.897 19.237 20.79326298 1.5 -7.293262983
Luc14 g048 1 0.028 0.016 1.798 27.867 14.35389529 1.5 -0.853895288
Luc14 g048 2 0.034 0.019 1.813 33.857 11.81439584 1.5 1.685604159
Luc14 g048 3 0.038 0.021 1.779 38.057 10.51054996 1.5 2.989450035

Not enough g025. Will start doing 100ul reactions since I reduced the number of cycles.