Haynes Lab:Notebook/CRISPR Editing/2016/04/12

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04/12/2016 transfected Luc14s with dCas9 g025, 31, and 34 using Neon, plated into 10cm plates Neon settings 3mL buffer E2 into Neon tube Pulse voltage 1,100 V Pulse width 20 Pulse # 2 Collect cells from flasks, need 5x10^6 cells / transfection Resuspended into 350ul Resuspension buffer R (ended up with too much volume, should have used less buffer?) mixed with 20ug DNA, used 100ul tips, plated immediately after transfection changes for next time use less resuspension buffer, or somehow modify so the final volume is right