Haynes Lab:Notebook/CRISPR Editing/2016/03/08

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03/08/2016

qPCR ChIP primer test
Results look good! Primers 6 and



Building dCas9_EGFP continued
PCR purified the amplified gB009 insert.

260/280 1.95
260/230 2.2
concentration 87.99 ng/ul

not as ugly as previous purifications
Cut 1ug per tube, two tubes (2ug total)

11.36 ul DNA
2 ul 10x cutsmart buffer
1 ul FseI
5.7 ul water

Incubated in PCR machine

30 min at 37degC
20 mins at 65degC

PCR purified

260/280 1.95
260/230 2.2
concentration 87.99 ng/ul