Haynes Lab:Notebook/CRISPR Editing/2016/02/26

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02/26/2016

PCR of gB014 gblock with P169/216, gotaq mm, at=55degC, 30 cycles, two 100ul rxns, ran on gel, confirmed both rxns had bands at the correct length
Screenshot 2016-03-12 20.22.52.png

Since the MV13-KAH60 digested and column purified backbone looked so badly on the nanodrop, I also ran it on the gel. still looks like the majority of the DNA is at the correct size though I did see some bands at lower MW.

passaged Luc14s thawed yesterday, look good.