Haynes Lab:Notebook/CRISPR Editing/2015/11/04

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11/04/2015

Ran PCR from 10/26/15 (Luc14 g031d g034d, g032) on gel

Nested PCR of 1:1000 diluted Luc14 g032 samples with P149/174
Ran on gel, bands in negative control, need to rerun.

PCR purified Luc14 g031d and g034d

Cell type gRNA donor rep 260 280 260/280 ng/µL
Luc14 g031 g031 EcoRI 1 0.035 0.019 1.894 35.13
Luc14 g031 g031 EcoRI 2 0.066 0.037 1.77 65.872
Luc14 g031 g031 EcoRI 3 0.058 0.032 1.792 58.161
Luc14 g034 g034 EcoRI 1 0.036 0.021 1.74 36.115
Luc14 g034 g034 EcoRI 2 0.029 0.016 1.739 28.507
Luc14 g034 g034 EcoRI 3 0.038 0.019 2.006 37.64