Haynes Lab:Notebook/CRISPR Editing/2015/10/26

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10/26/2015

gDNA extraction

Cell type gRNA donor rep 260 280 260/280 ng/µL
Luc14 g031 g031 EcoR1 donor 1 0.124 0.059 2.084 123.938
Luc14 g031 g031 EcoR1 donor 2 0.097 0.049 2 97.453
Luc14 g031 g031 EcoR1 donor 3 0.078 0.039 1.992 77.913
Luc14 g034 g034 EcoR1 donor 1 0.037 0.019 1.945 37.223
Luc14 g034 g034 EcoR1 donor 2 0.048 0.024 2.048 48.229
Luc14 g034 g034 EcoR1 donor 3 0.049 0.025 1.955 49.294
Luc14 g032 n/a 1 0.078 0.04 1.967 78.294
Luc14 g032 n/a 2 0.083 0.041 2.008 82.849
Luc14 g032 n/a 3 0.073 0.038 1.902 73.044



PCR with P197/198