Haynes Lab:Notebook/CRISPR Editing/2015/10/17

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10/17/2015

Luc14s looked great, 80-90% confluent. Proceeded with transfections.

Transfected in triplicate

750ng g031 in pX330GFP and 250ng g031 EcoRI donor PCR product
750ng g034 in pX330GFP and 250ng g034 EcoRI donor PCR product
500ng g032 in pX330GFP

Set up complexes, let form for 30 mins
Changed media to no antibiotics
Added complexes dropwise
centrifugation of the plate immediately post-transfection, 30 min @ 126 g and 37 °C.