Haynes Lab:Notebook/CRISPR Editing/2015/09/29

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09/29/2015

isolated gDNA from puro dox g032 g046 cells from a few weeks ago. Set up PCR with P197/198

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 200ng 5ul - 200ng
Gal4-EED puro g032 1 0.232 0.115 2.012 231.855 0.86 4.14
Gal4-EED puro g032 2 0.218 0.108 2.02 217.954 0.92 4.08
Gal4-EED puro g032 3 0.178 0.088 2.008 177.513 1.13 3.87
Gal4-EED dox g032 1 0.104 0.053 1.974 104.245 1.92 3.08
Gal4-EED dox g032 2 0.073 0.04 1.84 72.906 2.74 2.26
Gal4-EED dox g032 3 0.086 0.043 2.007 85.616 2.34 2.66
Gal4-EED puro g046 1 0.248 0.124 1.994 247.506 0.81 4.19
Gal4-EED puro g046 2 0.302 0.149 2.027 302.468 0.66 4.34
Gal4-EED puro g046 3 0.289 0.146 1.985 289.425 0.69 4.31
Gal4-EED dox g046 1 0.067 0.035 1.911 66.504 3.01 1.99
Gal4-EED dox g046 2 0.104 0.054 1.937 104.022 1.92 3.08
Gal4-EED dox g046 3 0.074 0.036 2.023 73.845 2.71 2.29

isolated gDNA from puro dox g054 g055 cells from a few weeks ago. Set up PCR with P197/198

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 200ng ul water to 10ul
Gal4-EED puro g054 1 0.033 0.014 2.289 32.879 6.08 3.92
Gal4-EED puro g054 2 0.026 0.012 2.232 26.294 7.61 2.39
Gal4-EED puro g054 3 0.028 0.013 2.252 28.36 7.05 2.95
Gal4-EED dox g054 1 0.069 0.033 2.102 69.023 2.90 7.10
Gal4-EED dox g054 2 0.07 0.034 2.091 70.196 2.85 7.15
Gal4-EED dox g054 3 0.061 0.03 2.052 61.451 3.25 6.75
Gal4-EED puro g055 1 0.166 0.082 2.033 166.248 1.20 8.80
Gal4-EED puro g055 2 0.225 0.114 1.981 224.964 0.89 9.11
Gal4-EED puro g055 3 0.19 0.093 2.039 190.432 1.05 8.95
Gal4-EED dox g055 1 0.1 0.05 2.021 100.183 2.00 8.00
Gal4-EED dox g055 2 0.115 0.055 2.079 114.683 1.74 8.26
Gal4-EED dox g055 3 0.117 0.057 2.07 117.461 1.70 8.30