Haynes Lab:Notebook/CRISPR Editing/2015/09/06

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09/06/2015

Finished gDNA extraction of 48h and 72h g034 gal4 dox or puro

Cell drug rep post tx 260 280 260/280 ng/µL ul for 200ng 5-ul 200
Gal4-EED puro 1 48h 0.041 0.019 2.156 40.577 4.93 0.07
Gal4-EED puro 2 48h 0.054 0.026 2.06 54.41 3.68 1.32
Gal4-EED puro 3 48h 0.052 0.023 2.228 51.71 3.87 1.13
Gal4-EED puro 1 72h 0.055 0.024 2.291 55.102 3.63 1.37
Gal4-EED puro 2 72h 0.063 0.032 1.974 62.666 3.19 1.81
Gal4-EED puro 3 72h 0.057 0.028 2.083 57.493 3.48 1.52
Gal4-EED dox 1 72h 0.117 0.057 2.039 117.084 1.71 3.29
Gal4-EED dox 2 72h 0.115 0.056 2.041 114.683 1.74 3.26
Gal4-EED dox 3 72h 0.075 0.035 2.159 74.776 2.67 2.33