Haynes Lab:Notebook/CRISPR Editing/2015/09/05

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09/05/2015

purified PCR products for luc14 g034 dilution experiment. anneal for surveyor

Cell type gRNA plasmid dil rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Luc14 g034 1ug 1 0.093 0.048 1.914 92.658 4.32 1.50 9.18
Luc14 g034 1ug 2 0.106 0.056 1.898 106.083 3.77 1.50 9.73
Luc14 g034 1ug 3 0.071 0.035 1.997 70.777 5.65 1.50 7.85
Luc14 g034 0.1ug 1 0.093 0.049 1.871 92.535 4.32 1.50 9.18
Luc14 g034 0.1ug 2 0.078 0.041 1.9 78.231 5.11 1.50 8.39
Luc14 g034 0.1ug 3 0.069 0.037 1.845 69.116 5.79 1.50 7.71
Luc14 g034 0.01ug 1 0.086 0.046 1.882 86.235 4.64 1.50 8.86
Luc14 g034 0.01ug 2 0.094 0.05 1.89 94.463 4.23 1.50 9.27
Luc14 g034 0.01ug 3 0.078 0.043 1.801 77.628 5.15 1.50 8.35



gDNA g034 time course exp purified 9/3, PCR with phusion P197/198

Cell type drug gRNA hours post tx rep 260 280 260/280 ng/µL ul for 200ng water to 31ul
Gal4-EED dox g034 24 1 0.018 0.007 2.551 18.406 10.87 20.13
Gal4-EED dox g034 24 2 0.017 0.008 2.132 17.049 11.73 19.27
Gal4-EED dox g034 24 3 0.015 0.005 3.02 15.337 13.04 17.96
Gal4-EED puro g034 24 1 0.038 0.019 2.028 37.537 5.33 25.67
Gal4-EED puro g034 24 2 0.034 0.016 2.127 34.405 5.81 25.19
Gal4-EED puro g034 24 3 0.029 0.013 2.164 28.746 6.96 24.04
Gal4-EED dox g034 48 1 0.033 0.015 2.19 32.784 6.10 24.90
Gal4-EED dox g034 48 2 0.039 0.018 2.141 39.299 5.09 25.91
Gal4-EED dox g034 48 3 0.029 0.016 1.85 28.83 6.94 24.06