Haynes Lab:Notebook/CRISPR Editing/2015/07/18

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07/18/2015

Finished gDNA extractions and first round pcr P197/198 on siRNA samples



Ligated a bunch of grnas into pX330g following Zhang lab protocol. transformed 5ul ligation into 100ul Ryan's z-comp DH5alpha turbos, incubated on ice for 30 mins, heat shock at 42deg for 35s, ice for 2min, plated all cells.

gRNA
gn001 T
gn001 B
gn003 T
gn003 B
g005
g0013
g057
g058
g059
g060