Haynes Lab:Notebook/CRISPR Editing/2015/07/08

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07/08/2015 Extracted gDNA from Luc14 0.1ug, 0.01ug, Gal4-EED+puro 1.0ug, and Gal4-EED+dox 1.0ug, 0.1ug, and 0.01ug samples. Ran PCR with P197/198 Luc14 1.0ug 0.1ug 0.01ug, Gal4-EED+puro 1.0ug Gal4-EED+dox 1.0ug 0.1ug 0.01ug Diluted PCR products 1:1000 fold, ran nested PCR with P163/215 Ran on gel, all looked good, nothing in the negative controls no template control from P197/198, negative control template with P163/215