Haynes Lab:Notebook/CRISPR Editing/2015/07/08

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07/08/2015

Extracted gDNA from Luc14 0.1ug, 0.01ug, Gal4-EED+puro 1.0ug, and Gal4-EED+dox 1.0ug, 0.1ug, and 0.01ug samples.
Ran PCR with P197/198

Luc14
1.0ug
0.1ug
0.01ug,
Gal4-EED+puro
1.0ug
Gal4-EED+dox
1.0ug
0.1ug
0.01ug


Diluted PCR products 1:1000 fold, ran nested PCR with P163/215
Ran on gel, all looked good, nothing in the negative controls

no template control from P197/198, negative control template with P163/215