Haynes Lab:Notebook/CRISPR Editing/2015/05/15
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Ran nested PCR reactions for dilution experiment and KAH228, GFP, g034 experiment done yesterday on a gel. All had correct bands and both the blanks were negative. Purified them using the Qiagen Qiaquick PCR purification kit.
Ran 2ul backbone on gel to confirm FseI activity. Saw one band at correct size for backbone, conclude FseI completely cut.
Put at -20°C