Haynes Lab:Notebook/CRISPR Editing/2015/05/14

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05/14/2015

nested PCR with P163 and P215 of Gal4-EED cells, no dox, cut with g034. Dilutions of plasmid and lipofectamine LTX reagent in duplicate

Cell type Rep ug plasmid ul lipo
Gal4-EED 1 0.5 3
Gal4-EED 2 0.5 3
Gal4-EED 1 1 3
Gal4-EED 2 1 3
Gal4-EED 1 0.5 5
Gal4-EED 2 0.5 5
Gal4-EED 1 1 5
Gal4-EED 2 1 5



nested PCR with P163 and P215 of reopening chromatin experiment with KAH228. I already did the first replicate so I'm only doing the second replicate now

1 Luc14 GFP g034 2
2 Luc14 GFP g034 3
3 Luc14 GFP KAH228 2
4 Luc14 GFP KAH228 3
5 Luc14 KAH228 g034 2
6 Luc14 KAH228 g034 3
7 Gal4-EED+dox GFP g034 2
8 Gal4-EED+dox GFP g034 3
9 Gal4-EED+dox GFP KAH228 2
10 Gal4-EED+dox GFP KAH228 3
11 Gal4-EED+dox KAH228 g034 2
12 Gal4-EED+dox KAH228 g034 3