 Project name
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03/24/2015
Ran on gel and did PCR purification kit for unt and g025 treated luc14 and gal4+dox cekks, nested PCR with P173/215.
Surveyor hybridization protocol on 200ng in 15ul
| Cell type
|
Treatment
|
Rep
|
260
|
280
|
260/280
|
ng/µL
|
ul for 200ng
|
ul hyb buff
|
ul water
|
| Luc14 |
Unt |
1 |
0.037 |
0.019 |
1.89 |
36.604 |
5.46 |
1.50 |
8.04
|
| Luc14 |
Unt |
2 |
0.025 |
0.014 |
1.745 |
25.137 |
7.96 |
1.50 |
5.54
|
| Luc14 |
Unt |
3 |
0.024 |
0.013 |
1.792 |
23.704 |
8.44 |
1.50 |
5.06
|
| Gal4-EED+dox |
Unt |
1 |
0.027 |
0.015 |
1.79 |
26.773 |
7.47 |
1.50 |
6.03
|
| Gal4-EED+dox |
Unt |
2 |
0.026 |
0.014 |
1.879 |
26.1 |
7.66 |
1.50 |
5.84
|
| Gal4-EED+dox |
Unt |
3 |
0.022 |
0.012 |
1.874 |
21.554 |
9.28 |
1.50 |
4.22
|
| Luc14 |
g025 |
1 |
0.023 |
0.012 |
1.897 |
23.003 |
8.69 |
1.50 |
4.81
|
| Luc14 |
g025 |
2 |
0.024 |
0.013 |
1.763 |
23.653 |
8.46 |
1.50 |
5.04
|
| Luc14 |
g025 |
3 |
0.023 |
0.014 |
1.649 |
22.935 |
8.72 |
1.50 |
4.78
|
| Gal4-EED+dox |
g025 |
1 |
0.023 |
0.013 |
1.76 |
23.111 |
8.65 |
1.50 |
4.85
|
| Gal4-EED+dox |
g025 |
2 |
0.018 |
0.01 |
1.765 |
17.939 |
11.15 |
1.50 |
2.35
|
| Gal4-EED+dox |
g025 |
3 |
0.026 |
0.016 |
1.59 |
26.08 |
7.67 |
1.50 |
5.83
|
Cut with 1ul Surveyor enzyme and 1ul enhancer. Ran on BA.
PCR purified
| Cell type
|
Treatment
|
rep
|
260
|
280
|
260/280
|
ng/µL
|
ul for 200ng
|
ul of hyb buff
|
ul water
|
| Luc14 |
Unt |
1 |
0.037 |
0.019 |
1.922 |
36.604 |
5.46 |
1.50 |
8.04
|
| Luc14 |
Unt |
2 |
0.03 |
0.016 |
1.84 |
30.185 |
6.63 |
1.50 |
6.87
|
| Luc14 |
Unt |
3 |
0.028 |
0.015 |
1.845 |
27.725 |
7.21 |
1.50 |
6.29
|
| Gal4-EED+dox |
Unt |
1 |
0.033 |
0.019 |
1.699 |
32.525 |
6.15 |
1.50 |
7.35
|
| Gal4-EED+dox |
Unt |
2 |
0.032 |
0.019 |
1.674 |
31.888 |
6.27 |
1.50 |
7.23
|
| Gal4-EED+dox |
Unt |
3 |
0.028 |
0.015 |
1.83 |
27.876 |
7.17 |
1.50 |
6.33
|
| Luc14 |
g044 |
1 |
0.028 |
0.015 |
1.818 |
28.126 |
7.11 |
1.50 |
6.39
|
| Luc14 |
g044 |
2 |
0.031 |
0.017 |
1.753 |
30.514 |
6.55 |
1.50 |
6.95
|
| Luc14 |
g044 |
3 |
0.03 |
0.017 |
1.732 |
30.199 |
6.62 |
1.50 |
6.88
|
| Gal4-EED+dox |
g044 |
1 |
0.025 |
0.014 |
1.766 |
25.351 |
7.89 |
1.50 |
5.61
|
| Gal4-EED+dox |
g044 |
2 |
0.032 |
0.017 |
1.81 |
31.522 |
6.34 |
1.50 |
7.16
|
| Gal4-EED+dox |
g044 |
3 |
0.034 |
0.02 |
1.696 |
33.517 |
5.97 |
1.50 |
7.53
|
| Luc14 |
g048 |
1 |
0.026 |
0.016 |
1.675 |
26.453 |
7.56 |
1.50 |
5.94
|
| Luc14 |
g048 |
2 |
0.018 |
0.011 |
1.648 |
18.227 |
10.97 |
1.50 |
2.53
|
| Luc14 |
g048 |
3 |
0.023 |
0.012 |
1.921 |
22.727 |
8.80 |
1.50 |
4.70
|
| Gal4-EED+dox |
g048 |
1 |
0.027 |
0.015 |
1.795 |
26.675 |
7.50 |
1.50 |
6.00
|
| Gal4-EED+dox |
g048 |
2 |
0.022 |
0.013 |
1.742 |
22.397 |
8.93 |
1.50 |
4.57
|
| Gal4-EED+dox |
g048 |
3 |
0.028 |
0.017 |
1.673 |
27.608 |
7.24 |
1.50 |
6.26
|
|
g023 and g046 gDNA extraction
| Cell type
|
Treatment
|
rep
|
260
|
280
|
260/280
|
ng/µL
|
ul for 200ng
|
| Luc14 |
g023 |
1 |
0.127 |
0.064 |
1.983 |
127.048 |
1.57
|
| Luc14 |
g023 |
2 |
0.107 |
0.055 |
1.948 |
106.752 |
1.87
|
| Luc14 |
g023 |
3 |
0.067 |
0.034 |
1.966 |
67.196 |
2.98
|
| Gal4-EED +dox |
g023 |
1 |
0.096 |
0.047 |
2.051 |
95.9 |
2.09
|
| Gal4-EED +dox |
g023 |
2 |
0.103 |
0.05 |
2.067 |
103.347 |
1.94
|
| Gal4-EED +dox |
g023 |
3 |
0.097 |
0.05 |
1.95 |
97.202 |
2.06
|
| Luc14 |
g046 |
1 |
0.151 |
0.077 |
1.97 |
151.192 |
1.32
|
| Luc14 |
g046 |
2 |
0.098 |
0.049 |
1.992 |
98.095 |
2.04
|
| Luc14 |
g046 |
3 |
0.093 |
0.047 |
1.969 |
93.4 |
2.14
|
| Gal4-EED +dox |
g046 |
1 |
0.135 |
0.069 |
1.959 |
135.41 |
1.48
|
| Gal4-EED +dox |
g046 |
2 |
0.086 |
0.043 |
2.002 |
86.375 |
2.32
|
| Gal4-EED +dox |
g046 |
3 |
0.08 |
0.042 |
1.922 |
79.87 |
2.50
|
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