Haynes Lab:Notebook/CRISPR Editing/2015/03/18

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03/18/2015

Ran four 50ul PCR reactions of 1:1000 diluted P197/198 Luc14 g031 rep 1. P149/174

ran on gel, single product of correct size.

purified PCR product, 2 columns, 50ul Es each, 100ul total:

Cell type treatment rep 260 280 260/280 ng/µL
Luc14 g031 1 0.119 0.063 1.874 118.594