03/12/2015
| Sample Read#
|
mL culture
|
elution
|
260
|
280
|
260/280
|
ng/µL
|
| KAH228 |
2 |
1 |
0.12 |
0.064 |
1.885 |
120.452
|
| KAH228 |
2 |
2 |
0.019 |
0.009 |
2.056 |
18.943
|
| KAH228 |
6 |
1 |
0.383 |
0.205 |
1.87 |
382.939
|
| KAH228 |
6 |
2 |
0.038 |
0.02 |
1.894 |
38.18
|
| KAH228 |
12 |
1 |
0.376 |
0.201 |
1.873 |
376.089
|
| KAH228 |
12 |
2 |
0.044 |
0.025 |
1.762 |
43.806
|
Cut 250ng of g031 treated luc14 and gal4eed+dox P151/158 nested PCR products. one hour at 30degC then heat inactivated at 65 for 20 minutes. No enzyme control has 13ul water, 0ul enzyme:
| water |
12.5
|
| DNA |
5
|
| Buffer |
2
|
| Bsa1I |
0.5
|
| total |
20
|
Ran on BA, saw lots of uncut amplicons, equal for Luc14 and Gal4, think the enzyme didn't cut completely.
|
Project name
Main project page
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