Haynes Lab:Notebook/CRISPR Editing/2015/03/10

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03/10/2015

Isolated genomic DNA from g025, g031, g032, and g044 treated Luc14 and Gal4-EED +dox cells.

Cell type gRNA Rep 260 280 260/280 ng/µL
Luc14 g025 1 0.202 0.102 1.989 202.49
Luc14 g025 2 0.206 0.104 1.974 206.149
Luc14 g025 3 0.215 0.109 1.976 214.727
Luc14 g031 1 0.174 0.089 1.961 174.274
Luc14 g031 2 0.243 0.123 1.974 242.751
Luc14 g031 3 0.186 0.094 1.972 185.631
Luc14 g032 1 0.153 0.077 1.981 153.231
Luc14 g032 2 0.177 0.09 1.973 177.378
Luc14 g032 3 0.127 0.064 2 127.353
Luc14 g044 1 0.186 0.095 1.96 186.258
Luc14 g044 2 0.191 0.097 1.972 190.951
Luc14 g044 3 0.139 0.071 1.958 138.566
Gal4-EED+dox g025 1 0.175 0.088 1.992 175.037
Gal4-EED+dox g025 2 0.206 0.105 1.967 206.458
Gal4-EED+dox g025 3 0.153 0.078 1.975 153.439
Gal4-EED+dox g031 1 0.165 0.085 1.947 165.133
Gal4-EED+dox g031 2 0.165 0.083 1.996 164.913
Gal4-EED+dox g031 3 0.137 0.068 2 136.788
Gal4-EED+dox g032 1 0.202 0.102 1.98 201.694
Gal4-EED+dox g032 2 0.216 0.11 1.965 216.26
Gal4-EED+dox g032 3 0.2 0.102 1.96 200.394
Gal4-EED+dox g044 1 0.195 0.099 1.966 195.174
Gal4-EED+dox g044 2 0.231 0.117 1.974 230.713
Gal4-EED+dox g044 3 0.222 0.113 1.953 221.679



Thawed more Luc14 and Gal4-EED cells to redo the opening chromatin experiment. Turns out I picked the KAH60 plasmid that's in a bacterial cloning vector and not a mammalian expression vector. Picked colonies from KAH228