03/10/2015
Isolated genomic DNA from g025, g031, g032, and g044 treated Luc14 and Gal4-EED +dox cells.
| Cell type
|
gRNA
|
Rep
|
260
|
280
|
260/280
|
ng/µL
|
| Luc14 |
g025 |
1 |
0.202 |
0.102 |
1.989 |
202.49
|
| Luc14 |
g025 |
2 |
0.206 |
0.104 |
1.974 |
206.149
|
| Luc14 |
g025 |
3 |
0.215 |
0.109 |
1.976 |
214.727
|
| Luc14 |
g031 |
1 |
0.174 |
0.089 |
1.961 |
174.274
|
| Luc14 |
g031 |
2 |
0.243 |
0.123 |
1.974 |
242.751
|
| Luc14 |
g031 |
3 |
0.186 |
0.094 |
1.972 |
185.631
|
| Luc14 |
g032 |
1 |
0.153 |
0.077 |
1.981 |
153.231
|
| Luc14 |
g032 |
2 |
0.177 |
0.09 |
1.973 |
177.378
|
| Luc14 |
g032 |
3 |
0.127 |
0.064 |
2 |
127.353
|
| Luc14 |
g044 |
1 |
0.186 |
0.095 |
1.96 |
186.258
|
| Luc14 |
g044 |
2 |
0.191 |
0.097 |
1.972 |
190.951
|
| Luc14 |
g044 |
3 |
0.139 |
0.071 |
1.958 |
138.566
|
| Gal4-EED+dox |
g025 |
1 |
0.175 |
0.088 |
1.992 |
175.037
|
| Gal4-EED+dox |
g025 |
2 |
0.206 |
0.105 |
1.967 |
206.458
|
| Gal4-EED+dox |
g025 |
3 |
0.153 |
0.078 |
1.975 |
153.439
|
| Gal4-EED+dox |
g031 |
1 |
0.165 |
0.085 |
1.947 |
165.133
|
| Gal4-EED+dox |
g031 |
2 |
0.165 |
0.083 |
1.996 |
164.913
|
| Gal4-EED+dox |
g031 |
3 |
0.137 |
0.068 |
2 |
136.788
|
| Gal4-EED+dox |
g032 |
1 |
0.202 |
0.102 |
1.98 |
201.694
|
| Gal4-EED+dox |
g032 |
2 |
0.216 |
0.11 |
1.965 |
216.26
|
| Gal4-EED+dox |
g032 |
3 |
0.2 |
0.102 |
1.96 |
200.394
|
| Gal4-EED+dox |
g044 |
1 |
0.195 |
0.099 |
1.966 |
195.174
|
| Gal4-EED+dox |
g044 |
2 |
0.231 |
0.117 |
1.974 |
230.713
|
| Gal4-EED+dox |
g044 |
3 |
0.222 |
0.113 |
1.953 |
221.679
|
Thawed more Luc14 and Gal4-EED cells to redo the opening chromatin experiment. Turns out I picked the KAH60 plasmid that's in a bacterial cloning vector and not a mammalian expression vector. Picked colonies from KAH228
| 
Project name
Main project page
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