Haynes Lab:Notebook/CRISPR Editing/2015/03/09

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03/09/2015

Restriction digest of KAH60 and pMaxGFP 6.2 to see why the KAH60-transfected cells aren't expressing mCherry. Cut about 0.5ug each, ran single cuts and uncut.

Lane 1 2 3 4 5 6 7 8
Plasmid KAH60 - 2 KAH60 - 2 KAH60 - 2 KAH60 - 1 pMax GFP 6.2 - 1 pMax GFP 6.2 - 1 pMax GFP 6.2 - 1 pMax GFP 6.2 - 2
Enzyme 1 N/A EcoRI EcoRI EcoRI N/A EcoRI EcoRI EcoRI
Enzyme 2 N/A N/A PstI PstI N/A N/A NotI NotI

I don't think I have the correct plasmid map for pMaxGFP. All lanes were the same uncut vector, correct size. KAH60 looks good.