Haynes Lab:Notebook/CRISPR Editing/2015/03/08

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03/08/2015

Checked GFP and mCherry fluorescence of pMaxGFP 6.2 or KAH60 transfected Luc14 and Gal4-EED cells.

pMaxGFP cells are green, both lines
took some nice images, can do flow tomorrow to get transfection efficiency
KAH60 cells are not red
will check plasmid with a digest tomorrow, maybe plasmid isn't there
will do flow tomorrow to see if it's just faint


will do luciferase assay to see if removing dox opens chromatin and if maybe KAH60 is opening chromatin just not red

Replaced media on gRNA transfected cells at 9pm, didn't wash