Haynes Lab:Notebook/CRISPR Editing/2015/03/02

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Induced Gal4-EED cells with dox.

I have P197/198 PCR products from Untreated, g046 treated, and g034 treated samples. I will dilute each of them 1:1000 fold and do nested PCR using primers P151 and P158. Annealing temp 68.

I will do PCR with P197/198 on g023, g045, and g048 samples.