Haynes Lab:Notebook/CRISPR Editing/2015/02/18

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02/18/2015

Ran test end-point PCR to determine if the qPCR primer sets I ordered to target pX330 will work for estimating transfection efficiency. Used gDNA from Luc14 treated with g023 rep 1 and g023 in pX330 as templates:

' For Rev
Primer set 1 P207 P208
Primer set 2 P209 P210
Primer set 3 P211 P212