Haynes Lab:Notebook/CRISPR Editing/2014/12/18

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12/18/2014

Ran PCR from yesterday on gel.
PCR purified g029 PCR reactions. Yield about 30ng/ul. PCR purified PCR reactions from yesterday. Some were as low as 23ng/ul. Will redo

Sample Read# 260 280 260/280 ng/µL
g029 0.032 0.016 2.007 31.813
g029 0.029 0.015 1.899 29.381
g023 0.033 0.02 1.674 33.467
g025 0.023 0.012 1.939 23.109
g030 0.023 0.012 1.862 22.585
untreated 0.023 0.013 1.839 23.346
all PCR for all gRNA treated cells, 2-50ul reactions gotaq at 61deg at.

Ran on gel
600
Extra bands on 31, 35, and 38. Will redo PCR at higher AT