Haynes Lab:Notebook/CRISPR Editing/2014/12/18

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12/18/2014 Ran PCR from yesterday on gel. PCR purified g029 PCR reactions. Yield about 30ng/ul. PCR purified PCR reactions from yesterday. Some were as low as 23ng/ul. Will redo Sample Read# 260 280 260/280 ng/µL g029 0.032 0.016 2.007 31.813 g029 0.029 0.015 1.899 29.381 g023 0.033 0.02 1.674 33.467 g025 0.023 0.012 1.939 23.109 g030 0.023 0.012 1.862 22.585 untreated 0.023 0.013 1.839 23.346 all PCR for all gRNA treated cells, 2-50ul reactions gotaq at 61deg at. Ran on gel Extra bands on 31, 35, and 38. Will redo PCR at higher AT