Haynes Lab:Notebook/CRISPR Editing/2014/12/17

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12/17/2014

Gradient PCR of g029 treated Luc14 gDNA. 6 temperatures, 50ul reactions.

53°C
55°C
57°C
59°C
61°C
63°C

Ran gel, only saw additional bands at 53°C, didn't see any drop off in yield.
Set up 50ul PCR reactions for g023, g025, g030, untreated luc14 gDNA. annealing temp 63.