Haynes Lab:Notebook/CRISPR Editing/2014/12/09

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12/09/2014

PCR of gRNA treated Luc14 gDNA for screening with Surveyor

' ul per 1 rxn
2x Taq MM 25
P197 100uM 2.5
P198 100uM 2.5
Sample 100ng/ul 2
H2O 18
Total 50


95°C for 3 min
30 cycles
95°C for 30 s
53°C for 30 s
72°C for 2 min
72°C for 10 min