Haynes Lab:Notebook/CRISPR Editing/2014/12/06

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12/06/2014

72 hours post transfection
Harvesting the cells (following Zhang lab recommendation for HEK 293 cells.
QIAamp genomic DNA extraction kit.

Sample Read# 260 280 260/280 ng/µL
g023 0.152 0.077 1.975 152.302
g025 0.146 0.073 1.991 146.123
g029 0.123 0.062 1.974 122.575
g030 0.111 0.056 1.998 111.309
g031 0.124 0.063 1.972 124.43
g032 0.136 0.069 1.983 136.408
g033 0.124 0.063 1.977 123.728
g034 0.139 0.071 1.968 138.986
g035 0.111 0.055 2.008 110.973
g036 0.125 0.064 1.971 125.365
g038 0.108 0.055 1.974 108.185
g039 0.09 0.047 1.928 90.499
g040 0.153 0.077 1.986 153.055
g041 0.127 0.065 1.961 127.455
g042 0.14 0.071 1.991 140.384
g043 0.126 0.064 1.962 125.746
g044 0.138 0.071 1.956 138.15
g045 0.151 0.077 1.97 150.75
g046 0.134 0.069 1.928 133.792
g048 0.13 0.066 1.965 130.029
g049 0.094 0.048 1.95 94.331
Untreated 0.111 0.056 1.998 110.915