Haynes Lab:Notebook/CRISPR Editing/2014/10/30

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10/30/2014

Picked colonies from transformation plates for gRNAs 26, 30, 39-49.

Minipreps for g033, g037, g038. Tested whether double eluting helps. I see these differences when reading the same sample twice. Can't conclude it makes any significant difference.

Sample Read# ' 260 280 260/280 ng/µL
g037 in pX330 single elution 0.025 0.014 1.789 25.032
double elution 0.029 0.016 1.807 28.52
g033 in pX330 single elution 0.162 0.085 1.907 162.222
double elution 0.164 0.086 1.91 164.041
g038 in pX330 single elution 0.028 0.014 1.963 27.505
double elution 0.031 0.017 1.873 30.949