Haynes Lab:Notebook/CRISPR Editing/2014/09/03

Project name

Main project page Previous entry      Next entry

09/03/2014 Spec'd Luc->AmCyan purified PCR products Sample Read# 260 280 260/280 ng/µL Luc->AmCyan PCR 3a 0.073 0.038 1.905 72.85 Luc->AmCyan PCR 3b 0.014 0.008 1.77 13.697 Luc->AmCyan PCR 4c worked. Purified using Sigma kit Sample Read# 260 280 260/280 ng/µL Luc->AmCyan PCR 4c 0.027 0.013 2.111 27.237 Ran PCR of PCR 4c (PCR 4c2), 100μL reaction, 2μL of each primer, 1μL sample. Sample Template F primer R primer Exp band 4c 2 4c P192 P195 918 Annealing Temp 55, et 1min Purified 4c 2 Sample Read# 260 280 260/280 ng/µL Luc->AmCyan PCR 4c 2 0.07 0.036 1.924 69.732