Haynes Lab:Notebook/CRISPR Editing/2014/09/03

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09/03/2014

Spec'd Luc->AmCyan purified PCR products

Sample Read# 260 280 260/280 ng/µL
Luc->AmCyan PCR 3a 0.073 0.038 1.905 72.85
Luc->AmCyan PCR 3b 0.014 0.008 1.77 13.697



Luc->AmCyan PCR 4c worked. Purified using Sigma kit

Sample Read# 260 280 260/280 ng/µL
Luc->AmCyan PCR 4c 0.027 0.013 2.111 27.237



Ran PCR of PCR 4c (PCR 4c2), 100μL reaction, 2μL of each primer, 1μL sample.

Sample Template F primer R primer Exp band
4c 2 4c P192 P195 918

Annealing Temp 55, et 1min
Purified 4c 2

Sample Read# 260 280 260/280 ng/µL
Luc->AmCyan PCR 4c 2 0.07 0.036 1.924 69.732