Haynes Lab:Notebook/CRISPR Editing/2014/09/02

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09/02/2014

Miniprepped colonies picked yesterday from ligation plates that were left out for 72 hours.

Sample Read# 260 280 260/280 ng/µL
gRNA 26 0.028 0.013 2.102 27.876
gRNA 30 0.022 0.008 2.556 21.644



Ran 3a and 3b PCR products on gel, both bands were there. 3a was really bright but both lanes had lots of background.
Purified the PCR products using Sigma kit.
PCR

Sample Template F primer R primer Exp band
4a 3a P179 P182 962
4b 3b P179 P182 962
4c 3b P192 P195 918

Annealing Temp 55, et 1min