Haynes Lab:Notebook/CRISPR Editing/2014/08/28

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Annealed gRNAs 26 and 30. Cut and ligated into pX330. Transformed into DH5αTs.

PCR cleaned the Luc-AmCyan PCR 1 from yesterday using Sigma PCR cleanup kit.
Second round of PCR

  • 2a - P185, P188
  • 2b - P190, P188

Ran on Wang lab machine, AT 44°C, ET 60s
Spun down liquid cultures started yesterday, put at 4deg.