Haynes Lab:Notebook/CRISPR Editing/2014/08/10

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08/10/2014

René did PCR clean ups, decent yield. double eluted in 40ul water.
René set up annealing of 400ng of PCR product in 15ul total volume (1.5ul of Phusion HF buffer).
Ash ran gel of purified PCR product
René set up Surveyor assay