Haynes Lab:Notebook/ASU iGEM/2012/08/14

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mm-dd-yy

Team Chimera

  • Insert work here


Team Cell Surface

  • Assembled magainin insert via Overlapping oligo assembly
  • Digested pUC 19 plasmid with EcoRI and PstI
  • Transformed Magainin insert into digested pUC 19 plasmid. Failed. Probably too much X-gal on plate.

Ran gel for the beta gal alpha-4 fragment. Failed. Fragment not in the correct size-band.

Alpha 4 re-PCR.jpeg