Haynes Lab:Notebook/ASU iGEM/2012/06/20

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6-20-12 Plated negative control on LB Amp plate Liquid cultures of T7 promoter and constitutive promoter Transformation (LSE) Transformed DNA: RBS (well 1:1H BBa_B0030) TetR GFP (well 2:8A Part:BBa_I13522) Cells: DH5 alpha (donated) Protocol from: http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf (30 minute transformation) Controls: puc19, no DNA