Haynes Lab:Notebook/ASU iGEM/2012/06/13

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6-13-12 Transformation (LSE) Transformed DNA: lacZ (well 4:12G, I732019) p + lacO (well 1:6G, R0011) Cells: DH5 alpha (donated) Protocol from: http://openwetware.org/wiki/Haynes:Assembly101 (30 minute transformation) Controls: puc19, no DNA (8 plates) Transformation (istb4, Abhi) Transformed DNA: Cells: Protocol from: Controls: DH5a Chemically Competent cell prep Grew 2 seed colonies from streak plate in LB no amp Grew controls to test for contamination Both Seed colonies grew, no contamination present