G.tigrina Hox gene DthoxC insertion into prokaryote E.coli / (by UNIamCloning)/2011/10/13
|Project name||Main project page|
Previous entry Next entry
PCR Check of Sample Gt.5-2
DNA samples were run at various annealing temperatures in the ThermoGradient using both sets of primers. The annealing temperatures tried were: 51.0°C, 50.3°C, 47.9°C, 45.4°C, 44.0°C. These temperatures were chosen because 44.0°C was the ideal annealing temperature for COI-1 and COI-2 (as well as in a nice range for 15_DthoxCa_F and 15_DthoxCb_R)and we stopped at 51.0°C to avoid the melting point of the DthoxC primers. --The results of this amplification will be tested via electrophoresis on Tuesday!