File:PCR BbGibson tests 080611.jpg

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Agarose gel of PCR reactions amplifying standard Biobrick vectors and some parts with new bbGibson primers. Stained with EZ-vision (Amresco). Lane 1. Mass Ruler (Fermentas), 2. pSB1T3 (linear PCR template) V.f/V.r, 3. pSB1C3 (circular plasmid template) V.f/V.r, 4. I13522 (tetR-RBS-GFP) A.f/ END.r, 5. I13522 (tetR-RBS-GFP) A.f/ A.r, 6. I13521 (tetR-RBS-RFP) B.f/ END.r.

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current00:48, 15 August 2011Thumbnail for version as of 00:48, 15 August 20111,600 × 1,200 (185 KB)Andrew Perry (talk | contribs)Agarose gel of PCR reactions amplifying standard Biobrick vectors and some parts with new bbGibson primers. Stained with EZ-vision (Amresco). Lane 1. Mass Ruler (Fermentas), 2. pSB1T3 (linear PCR template) V.f/V.r, 3. pSB1C3 (circular plasmid template) V.
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