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The experimental process of recombinant lentivirus protocol production is as follows. 1. Obtain the target genes by PCR. 2. Choose the corresponding lentivirus vector plasmid according to your requests. 3. Construct recombinant lentivirus plasmid. 4. Identify the insert gene sequence, and then extract recombinant lentivirus plasmids. 5. Seed 293T packaging cells at 1.3-1.5 X 105 cells/ml (6 ml per plate) in Seeding media (DMEM + 10% FBS without Penicillin / Streptomycin) in culture plates. Incubate cells for 24 h (37 °C, 5% CO2), or until the following afternoon. After ~24 h, the cells should be ~70% confluent. ...