File:Kinetic Characterization of Atl1 Affinity for ODNs.jpg

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Kinetic measurements of binding between Atl1 and ODN (A) Steady state kinetics using ELISA. Increased concentrations of Atl1 incubated with biotin tagged ODN bound to streptavidin microtiter plate.(B) IC50 values from the direct ELISA curves.(C) SPR sensorgrams for the indicated ODN.(D) SPR-determined interaction constants ka (association on rate), kd (dissociation [on] rate), and KD (dissociation [off] constant).

Latypov, V. F., et al. (2012). "Atl1 regulates choice between global genome and transcription-coupled repair of O(6)-alkylguanines." Mol Cell 47(1): 50-60.

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current07:28, 26 February 2013Thumbnail for version as of 07:28, 26 February 2013506 × 715 (141 KB)Tanya E Raymond (talk | contribs)Kinetic measurements of binding between Atl1 and ODN (A) Steady state kinetics using ELISA. Increased concentrations of Atl1 incubated with biotin tagged ODN bound to streptavidin microtiter plate.(B) IC50 values from the direct ELISA curves.(C) SPR sens
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