Fig. 1. Scheme of the antigen-mediated genetically modified cell amplification (AMEGA) system. (A) The construct and the activation scheme of the chimeric receptor. The extracellular N-terminal half of EpoR was replaced with ScFv of anti-fluorescein antibody 31IJ3 with a GSG tripeptide linker between ScFv and Val 118 of EpoR D2 domain to create ScFvg. VH and VL regions were linked with a S(G4S)3 flexible linker. Fluorescein dimer addition brings two ScFvg chains into close proximity, leading to signal transduction. (B) Schematic diagram of the selection procedure. Retroviral vectors with long-terminal repeats (LTRs) and a packaging signal () are used for the efficient transcription of ScFvg-IRES-transgene. An immunoglobulin heavy chain secretion signal sequence (S) is located upstream of the chimeric receptor gene to enable their cell surface expression. -from the paper
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