Etchevers:Notebook/STRA6 in eye development/2008/08/09

Genetics of human eye development

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Getting reagents together Stan Faguer had received his kits, was hoping to avoid nucleus isolation so still has not found out about ultracentrifuge availability. The kit had been frozen; Roche confirmed by telephone that the protein-A agarose probably loses its properties. Still need to order IgG. We have a couple of mL of prot A agarose still in the fridge. Am still tempted by the Dynal bead protocol but I can’t believe it makes that big a difference. Need to closer examine the protocols for amplication of target to be sequenced on the Roche 450. Looked into doing it on the Illumina system in Denmark with Niels Tommerup (cf. seminar notes) Alethea 11:20, 9 August 2008 (UTC):