Etchevers:Notebook/Genomics of hNCC/2009/03/24
|Genomics of human neural crest cells||Main project page|
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The hNCC from R1113 are doing very well and seem to have divided once in the dish, or at least spread to make whorls in the 35mm dishes. Counts on average for both 35mm dishes = 540K/2 cells = 270,000? seems few, given density.
Plated into two collagen-coated 25 cm2 flask.
For the non-coated plastic, 400K/2 hNCC cells or 200,000 cells. Plated into non-coated 25 cm2 flask.
Trypsined also all the neural differentiation cultures. Lots of cells recovered from the vitamin A + extra EGF cultures, clonal density for the FGF2/ extra EGF medium. Stopped trypsin in 10% FCS/DMEM but recultured in their non-serum-containing medium.