Etchevers:Notebook/Genomics of hNCC/2009/03/23
|Genomics of human neural crest cells||Main project page|
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Survival and plans for the week
All cells looked good, even those plated on non-collagen dishes (but these like all the others look pretty actin-fiber-filled). Some cell death but not over-much.
Should be working in quarantine now because re-thawed cells, until the mycoplasma test is finished.
Rat tail collagen from BD Biosciences, ref 354236 lot 32334. This is at 3.92 mg/mL. Should dilute to 50 μg/mL. This is a factor of dilution = 78.4. Dilute in PBS, though BD suggests in 0.02N acetic acid? (Millipore in 0.01N and I think last time, I used PBS. No wonder it agglomerated. Used 0.02N acetic acid in the end.)
Ordered GP Express Plus membrane 0.22μm 150 mL receiver bottle cat no. SCGPU01RE from the magasin, as well as stuff to clean computers. Did MB work for the genomics/eye project.