Etchevers:Notebook/Genomics of hNCC/2009/03/16

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Neural differentiation cultures

Fed the four flasks and four coverslips with fresh B27/Stemline Neural medium with EGF+FGF2 and/or EGF+retinyl ester. Had added 5 mL to regular 3 mL in flasks Friday afternoon.

One of the EGF+retinyl ester flasks seems to have a lot of fasciculating neurites in it! But the coverslips don't for the moment, although individual cells look like potential neurons. Added the EGF+FGF2 medium to the two wells with Rich medium that have been going since forever.

  • Heather 12:25, 16 March 2009 (EDT):