Etchevers:Notebook/Genomics of hNCC/2008/11/13

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C14.jpg Genomics of human neural crest cells <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Fluorescence microscope pictures by myself

  • Heather 11:17, 13 November 2008 (EST):

I also wrote about these a little in today's blog post. All these photos are of cephalic human neural crest cells discussed over the last couple of months in the notebook. Also check out this link for cell compartments (ER vs Golgi?).

  • hEScGro medium

HEScGRO ceph C-S red 40x 3 small.jpg alpha SMA (red, Alexa Fluor 555); CNPase not very visible from brief passage on confocal. Look again on Tuesday.

HEScGRO ceph C-S all 40x flat.jpg alpha SMA (red, Alexa Fluor 555), superimposed with green/blue autofluorescence.

  • C6000 CHO cell medium

C6000 ceph tB all 4-6s 3-1 gain 40x flatjpg.jpg TuJ1 (invisible at Alexa Fluor 633) + beta 2 microglobulin (Alexa Fluor 555), superimposed with green/blue autofluorescence.

C6000 ceph C-S 40x red 2 290ms 3-1x gain.jpg alpha SMA (red, Alexa Fluor 555)

C6000 ceph C-S 40x blue flat.jpg alpha SMA (red, Alexa Fluor 555), superimposed with blue autofluorescence only.

  • Stempan stem cell medium (PAN)

Stempan ceph C-S all 20x 2s 3-1 gain flat.jpg alpha SMA (red, Alexa Fluor 555), superimposed with green/blue autofluorescence. 20x instead of others at 40x.

Stempan ceph C-S red 20x 2s 3-1 gain 2.jpg alpha SMA (red, Alexa Fluor 555), 20x.

Stempan-N ceph t-B all 40x 2 2s 3-1 gain flat.jpg TuJ1 (invisible at Alexa Fluor 633) + beta 2 microglobulin (Alexa Fluor 555), superimposed with green/blue autofluorescence.

Stempan ceph PGD2 all 40x 2 2s 3-1 gain.jpg PGD2 doesn't seem to stain much (in red, Alexa Fluor 546). Red, green and blue perfectly superimpose. 40x.

Aggrandiss Stempan ceph C-S all 40x copier.jpg alpha SMA (red, Alexa Fluor 555), superimposed with green/blue autofluorescence. Note vesicles, as if secreted from Golgi eg. lysosomes, peroxisomes or melanosomes.

  • 401 Panserin medium. The one with all the melanocyte-like things with phase-dark vesicles. Anything marked "nucleus" refers to a non-stained oval structure.

401 ceph C-S red 40x 6 2s gain 3-1 small.jpg alpha SMA (red, Alexa Fluor 555), 40x.

401 ceph C-S red 40x 3 2s gain 3-1 flat.jpg alpha SMA (red, Alexa Fluor 555), 40x.

401 ceph C-S red 40x 2 2s gain 3-1.jpg alpha SMA (red, Alexa Fluor 555), 40x.

  • Heather 11:17, 13 November 2008 (EST):