Etchevers:Notebook/Genomics of hNCC/2008/10/22

From OpenWetWare
Jump to: navigation, search
C14.jpg Genomics of human neural crest cells Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Petit bout de papier

Looked at rabbit cornea for the male 980; it may have been the control rabbit; the R and L corneas looked identical. Some auto-fluorescence in collagen layers (PAF fixed, no anti-aldehyde treatment), scar not obvious to find in either case either. May need to cut more eyes, may need to deparaffinate - but I don't think so.

Took photos of different cultures after fixation on light microscope.

401 20x1.JPG Panserin 401 medium. Cephalic hNCC at 20x.

401 20x2.JPG Panserin 401 medium. Cephalic hNCC at 20x.

C6000 20x.JPG CHO C6000 medium. Cephalic hNCC at 20x.

H-ceph-20x.JPG hEScGro medium. Cephalic hNCC at 20x.

H-T 20x1.JPG hEScGro medium. Truncal hNCC at 20x.

H-T 20x2a.JPG hEScGro medium. Truncal hNCC at 20x, another shot.

H-T 20x2b.JPG hEScGro medium. Truncal hNCC at 20x, another shot.

S-ceph0.JPG Stempan medium. Cephalic hNCC at 20x.

S-ceph1 20xarrows.jpg Stempan medium again, Cephalic hNCC at 20x, arrows showing spaces cells seem to avoid on plastic.

S-ceph2-20xarrow.jpg Stempan medium again, Cephalic hNCC at 20x, arrow showing an apparent neurite or other ramification.

S-ceph4-20x futureN.JPG Stempan medium then 3 days of neural differentiation medium from Sigma. Cephalic hNCC at 20x.

S-ceph5-20x futureN.JPG Stempan medium then 3 days of neural differentiation medium from Sigma. Cephalic hNCC at 20x.

  • Heather 05:55, 7 November 2008 (EST):