Erickson lab:Notebook/defensin expression/2008/07/29

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Redo of PCR with different Primers

  • Rerun of PCR using colonies 1-16 from 7/22 and primers TOPOF and TOPOR


10μL Master Mix (Econo plus green)

2μL each primer(5uM)

6μL water

bacteria colonies 1-16, pos. & neg. controls

Ran a gel of the PCR products

File:Cat flea PCR 7.29.08B.TIF

     Lane 1: 2-Log Ladder
     Lane 2-17: samples 1-16
     Lane 18-19: controls